Adenosine, a purine nucleoside, is present throughout the body and plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and regulates the Renin-Angiotensin system. Adenosine also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure.
Adenosine Assay Kit (Fluorometric) is used to detect adenosine in plasma and urine, the adenosine is measured by combining adenosine deaminase with a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the adenosine probe, leading to the formation of a fluorescent product. The fluorescent product can be detected at Ex/Em = 535nm/587 nm, and its intensity is proportional to the amount of adenosine in the sample. The detection range of this product is 2-80 pmol of adenosine in plasma or urine.
- 25 ml Adenosine Assay Buffer
- 1 vial Adenosine Convertor (10 U)
- 1 vial Adenosine Detector (10 U)
- 1 vial Adenosine Developer (1 U)
- 200 µl Adenosine Probe (in DMSO)
- 100µl Adenosine Standard (10 mM)
- 1 vial Urine Clarifier (1 U)
Store kit at -20°C in the dark immediately upon receipt. Kit is stable for 1 year from receipt. Reconstituted components are stable for 2 months.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.