The Celltechgen BCA (bicinchoninic) protein assay is a widely used method for colorimetric Copper-based detection and quantitation of total protein in a solution. The BCA assay involves two steps. First is the biuret reaction, whose faint blue colour results from the reduction of cupric ion to cuprous ion. Second is the chelation of BCA with the cuprous ion, resulting in an intense purple colour. The purple coloured reaction product is formed by the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water-soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. BCA is sensitive and has a broad dynamic range; capable of measuring protein concentrations of 0.5 μg/mL to 1.5 mg/mL.
The advantage of BCA is that the reagent is fairly stable under alkaline conditions, and can be included in the copper solution to allow a one-step procedure. One of the biggest benefits of this method is that the BCA protein assay is compatible with most protein samples and protein samples that contain up to 5% surfactants (detergents). In addition, the BCA Assay responds more uniformly to different proteins than the Bradford method. However, Reducing reagents, certain single amino acids (such as cysteine or cystine, tyrosine and tryptophan) and Reagents that chelate the copper can produce colour and interfere in BCA assays.
Sufficient For: 500 tube assays or 5000 microplate assays
- BCA Reagent A, 2 x 500 mL
- BCA Reagent B, 25 mL
- Albumin Standard Ampules, 2mg/mL, 10 x 1 mL
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards and safe handling practices.
Store at room temperature.