Biotinylated Protein L

Biotinylated Protein L

Introduction:

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Recombinant Protein L Protein, fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. DTT-reduced protein migrates as a 36 to 38 kDa polypeptide.

Biotinylated Protein L Contains four immunoglobulin-binding domains per protein, and binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains, single chain variable fragments (scFv) and Fab fragments that contain kappa light chains.

Size: 0.5 mg

Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Specificity:
Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies.

Storage: Avoid repeated freeze-thaw cycles, in lyophilized state for 1 year store at 4^oC-8^oC for 1 year in lyophilized state; store at 4^oC-8^oC for 1 month after reconstitution under sterile conditions or 3 months at -20^oC to -70^oC.

Usage:
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

IgG Binging to Protein A, G and L:

Strong binding ＋＋, medium interaction ＋, weak or no interaction －.