Caspase-5 Fluorometric Assay Kit
The Caspase-5 Fluorometric Assay Kit is designed to provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on detection of cleavage of substrate WEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). WEHD-AFC emits blue light (Emax = 400 nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (Emax = 505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
WEHD-AFC Substrate: 500 μL
1 M dithiothreitol (DTT): 400 μL
Lysis Buffer: 100 mL
2X Reaction Buffer: 10 ml
Store at ≤ -20 °C for up to 6 months after initial use. Avoid repeated freeze-thaw cycles.
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