The CFSE Cell Proliferation Kit contains carboxyfluorescein diacetate, succinimidyl ester (CFSE) in ten singleuse vials. Small-scale experiments can be performed without preparing excess quantities of perishable CFSE stock solution, a high-quality DMSO (dimethylsulfoxide) and a detailed protocol are also included. When CFSE passively diffuses into cells, it is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield highly fluorescent carboxyfluorescein succinimidyl ester. The succinimidyl ester group reacts with intracellular amines, forming fluorescent conjugates that are well retained and can be fixed with aldehyde fixatives. Excess unconjugated reagent and by-products can be washed away. The dye–protein adducts that form in labeled cells are retained by the cells throughout development and meiosis, and can be used for in vivo tracing. The label is inherited by daughter cells after either cell division or cell fusion, and is not transferred to adjacent cells in a population. Mouse cells labeled with CFSE have been detected up to eight weeks after injection into mice body.
The approximate excitation and emission peaks of this product after hydrolysis are 492 nm and 517 nm, respectively. Cells labeled with CFSE can be visualized by fluorescence microscopy using standard fluorescein filter sets or analyzed by flow cytometry in an instrument equipped with a 488 nm excitation source.
500ug ; 5 mg ; 25mg
CFSE (Component A),
DMSO (Component B),
Upon receipt, components should be stored desiccated at ≤–20°C until required for use. AVOID REPEATED FREEZING AND THAWING. Before opening the vial, allow the product to warm to room temperature. When stored properly, both the DMSO and solid CFSE should be stable for at least six months. Solutions of the reagent should be used promptly.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.