Recombinant Mouse Leukemia inhibitory factor
Recombinant mouse leukemia inhibitory factor (LIF) is a bioactive protein intended for use in cell culture applications. LIF is known for its ability to maintain the pluripotency of mouse embryonic stem cells. LIF is also involved in a wide variety of biological processes, including hematopoietic cell proliferation, neuronal survival and formation, and bone formation.
Expression: E. coli
Gene Aliases: LIF
Gene ID: 16878
Gene Symbol: LIF
Molecular Weight ~20.0 kDa (181 amino acids)
Protein Subtype: Other Cytokines
Protein Tag or Fusion: Untagged
Validated Application: Cell Culture
Purity ≥95% as determined by SDS-PAGE analysis.
Mouse LIF biological activity is determined by the ability to induce IL-6 production in M1 myeloid leukemic cells. The ED50 of mouse LIF in this assay is ≤1ng/mL.
Lyophilized from a sterile (0.2 μm) filtered aqueous solution containing 0.1% trifluoroacetic acid (TFA), Reconstitution Sterile 10mM Acetic Acid at 0.1mg/ml. Centrifuge vial before opening. Do not vortex. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeated freeze thaws.
Store at -20°C – -80°C for 12 months, 1 month at 4°C after reconsituted or 3 months when stored at -20°C – -80°C after reconstituting as directed.