Protein A/G Agarose
Catalogue #: P02-P0115
Size: 1 ml (settled), Support: Crosslinked 6% beaded agarose supplied as a 50% slurry (e.g., 1mL of settled resin is equivalent to 2 mL of 50% slurry).
Binding capacity: 27mg mouse IgG or ≥ 50mg human IgG/mL
Description: Approximately 50.5 kDa. Recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to Protein A and G domains that are included in the recombinant sequence. Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. Protein G portion is from Streptococcus segments C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.
Specificity: The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.
Applications: Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
Formulation: Solution in 20% alcohol. Before use, please spin down the beads, move away the supernatant, wash with PBS or the desired buffer three times, keep at 4-8C.
Storage: 1 year at 2-8°C. Don’t freeze. Product is shipped at ambient temperature.
Usage: This material is for research, laboratory or further evaluation purposes.