Protein A/G Magnetic Beads
Catalogue #: P02-P01010
Size: 1mL, supplied at 10mg/mL in PBS containing 0.05% Tween™-20 Detergent and 0.05% NaN3. Recombinant Protein G covalently coupled to magnetic bead surface (10mg/mL). Binding Capacity is 55 to 85µg rabbit IgG per mg
Molecular Weight: Approximately 50.5 kDa. Recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to Protein A and G domains that are included in the recombinant sequence. Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. Protein G portion is from Streptococcus segments C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.
Biological Activity: The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of
thrombin. The specific activity is no less than 10,000ATU/mg protein.
Specificity: The extended Fc-binding properties of Protein A/G make it a popular tool in the
investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.
Applications: Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
Storage: 1 year at 2-8°C. Don’t freeze. Product is shipped with an ice pack.
Usage: This material is for research, laboratory or further evaluation purposes.