Protein G Magnetic Beads
Catalog Number: P02-P0111M
Size: 1mL, supplied at 10mg/mL in PBS containing 0.05% Tween™-20 Detergent and 0.05% NaN3. Recombinant Protein G covalently coupled to magnetic bead surface (10mg/mL). Binding Capacity is up to 60µg rabbit IgG/mg
Description: Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.
Specificity: Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.
Applications: The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2.
Storage: 1 year at 2-8°C. Don’t freeze. Product is shipped with an ice pack.
Usage: This material is for research, laboratory or further evaluation purposes.