Protein G, R-PE Conjugated
Catalog Number: P02-P0111R
Size: 100 µg in 0.1 ml
Description: Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.
Phycoethyrin (R-PE): R-PE is a 240 kDa protein from the light-harvesting complex, which is composed of a hexameric structure of alpha and beta chains andphycobilins. R-PE purified from Poryhyratenera.
Protein G, R-PE conjugate is a protein labled by Phycoethyrin.
Formulation: 100 µg of Protein A conjugated with R-PE in PBS.
Excitation Laer: Blue Laser (488 nm)/Green Laser (532 nm)/Yellow-Green Laser (566 nm)
Specificity: Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.
Applications: The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2.
Immunofluorescence (IF) 1: 50-1:200
Flow cytometry 1: 100-1:500.
Storage: 1 year at 2-8°C. Don’t freeze. Product shipped with an ice pack.
Usage: This material is for research, laboratory or further evaluation purposes.