Recombinant Human Caspase-10 (Mch4)

Recombinant Human Caspase-10 (Mch4)

Size: 10 ug

Source

E. Coli, Val220-­Ile479, with a C-­terminal 6­-His tag

Accession number

AAC50644

N-­terminal Sequence Analysis

Val220 & Ala373

Molecular Weight

17 kDa & 10 kDa, under reducing and non­reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate N­acetyl­Leu­Glu­His­Asp­7­amino­4­trifluoromethyl coumarin (Ac­LEHDAFC). The specific activity is > 200 pmol/min/µg, as measured under the described conditions.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Purity

95%, by SDS­PAGE.

Formulation

Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT. See Certificate of Analysis for details.

Shipping & Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. Avoid repeated freeze/­thaw cycles. Store at ­70 °C for 6 months from date of receipt, store ­at 70 °C under sterile conditions after opening for 3 months.