Recombinant Human Caspase-10 (Mch4)
Size: 10 ug
Source
E. Coli, Val220-Ile479, with a C-terminal 6-His tag
Accession number
AAC50644
N-terminal Sequence Analysis
Val220 & Ala373
Molecular Weight
17 kDa & 10 kDa, under reducing and nonreducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate NacetylLeuGluHisAsp7amino4trifluoromethyl coumarin (AcLEHDAFC). The specific activity is > 200 pmol/min/µg, as measured under the described conditions.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Purity
95%, by SDSPAGE.
Formulation
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT. See Certificate of Analysis for details.
Shipping & Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. Avoid repeated freeze/thaw cycles. Store at 70 °C for 6 months from date of receipt, store at 70 °C under sterile conditions after opening for 3 months.
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