Recombinant Human Caspase-3
Size: 10 ug
E. coli, Ser29-Asp175 (subunit 1) & Ala183-His277 (Asp190Glu) (subunit 2)
N-terminal Sequence Analysis
Ser29 (subunit 1) & Ala183 (subunit 2)
18 kDa and 10 kDa, reducing conditions (SDS-PAGE)
Measured by its ability to cleave the fluorogenic peptide substrate AcDEVDAFC. The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
<1.0 EU per 1 μg of the protein by the LAL method.
95%, by SDSPAGE.
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Sucrose. See Certificate of Analysis for details.
Shipping & Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. Avoid repeated freezethaw cycles. Store at 70 °C for 6 months from date of receipt, store at 70 °C under sterile conditions after opening for 3 months.