Recombinant Human Caspase 8
Size: 10 ug
Source
E. coli, Ser217-Asp384 (Asp285His) (p18 subunit) & Leu385-Asp479 (p10 subunit), with a C-terminal 6-His tag
Accession number
Q14790
N-terminal Sequence Analysis
Ser217 (p18 subunit) & Leu385 (p10 subunit)
Molecular Weight
18 kDa & 10 kDa, reducing conditions (SDS-PAGE)
Activity
Measured by its ability to cleave a fluorogenic peptide substrate AcIETDAFC. The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Purity
95%, by SDSPAGE.
Formulation
Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT. See Certificate of Analysis for details.
Shipping & Storage
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. Avoid repeated freeze/thaw cycles. Store at 70 °C for 6 months from date of receipt, store at 70 °C under sterile conditions after opening for 3 months.
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