Recombinant Human Caspase 8

Recombinant Human Caspase 8

Size: 10 ug

Source

E. coli­, Ser217-­Asp384 (Asp285His) (p18 subunit) & Leu385-­Asp479 (p10 subunit), with a C­-terminal 6-­His tag

Accession number

Q14790

N-­terminal Sequence Analysis

Ser217 (p18 subunit) & Leu385 (p10 subunit)

Molecular Weight

18 kDa & 10 kDa, reducing conditions (SDS-PAGE)

Activity

Measured by its ability to cleave a fluorogenic peptide substrate Ac­IETD­AFC. The specific activity is >1,000 pmol/min/μg, as measured under the described conditions.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Purity

95%, by SDS­PAGE.

Formulation

Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT. See Certificate of Analysis for details.

Shipping & Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. Avoid repeated freeze/­thaw cycles. Store at ­70 °C for 6 months from date of receipt, store ­at 70 °C under sterile conditions after opening for 3 months.