Rhod-2 AM has the longest fluorescent emission signal of the commonly used calcium indicators. It contains a rhodamine-like fluorophore, making it suitable for use with argon and krypton laser excitation sources. Rhod-2 signal intensity one of the strongest of all the calcium probes. Rhod 2 is especially suitable probe for intracellular calcium monitoring by confocal laser-scanning microscopy and flow cytometry. The dissociation constant of Rhod 2 with calcium is among the highest of all the fluorescent calcium probes, providing a wider range for monitoring calcium concentration. Rhod-2 AM is an acetoxymethyl ester derivative of Rhod-2 that can be easily loaded into cells by passive incubation. Wavelength Maxima: Excitation 549nm, Emission 578nm.
Purity > 93%
CAS Number 145037-81-6
Chemical name 9-[4-[Bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy) methoxy]-2-oxoethyl] amino]-5-methylphenoxy]ethoxy] phenyl]-3,6-bis (dimethylamino) xanthylium bromide
Molecular weight 1123.96
Molecular formula C52H59BrN4O19
Solubility Soluble in DMSO to 1 mg/ml
50 ug ; 5*50ug
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. Handling Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month.
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