Catalog Number: AX-AP0016
Size: 0.1 mg (0.2 mg/ml)
Absorption Maximum: 496 nm
Emission Maximum: 667 nm
Streptavidin, R-Phycoerythrin/Cy5 conjugate, also call tamdem RPE streptavidin, is a fluorescent streptavidin conjugate that has broad applications in cytometry and luminex bead based assay. These reagents can also be employed to localize biocytin, biocytin-X, biotin ethylenediamine and so on.
Supplied: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
A biotinylated primary molecules such as an antibody is bound to tissues, cells, or other surfaces. Excess protein is removed by washing, and detection is mediated by adding Streptavidin-R-Phycoerythrin/Cy5. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the R-PE/Cy5 conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Store Streptavdin, R-PE/Cy5 conjugate in dark at 2~8oC. Do not freeze. For research or further manufacturing use only.