The Celltechgen™ M-MuLV Reverse Transcriptase (200 U/µL) is a genetically modified M-MuLV RT. It differs from wildtype MMuLV RT by its structure, catalytic properties and in the optimum activity temperature. The enzyme possesses RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastis Virus (AMV) reverse transcriptase (1,2). RevertAid™ Reverse Transcriptase activity is optimal at 42°C (active up to 50°C). The enzyme is capable of first strand cDNA synthesis up to 13 kb. The enzyme incorporates modified nucleotides.
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR, see protocol on back page.
• Synthesis of cDNA for cloning and expression.
• Generation of labeled cDNA probes for microarrays.
• DNA labeling (3).
• Analysis of RNA by primer extension (3).
Store at -20℃
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.