The Cytochrome c Oxidase Assay Kit is designed for the determination of cytochrome c oxidase activity in soluble and membrane bound mitochondrial samples. Cytochrome c oxidase is the last enzyme in the respiratory electron transport chain of mitochondria. Its main function is to convert molecular oxygen to water and aid in establishing mitochondrial membrane potential. Cytochrome c oxidase locates to the inner membrane which separates the mitochondrial matrix from the intermembrane space. This colorimetric assay is based on observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for detection of mitochondrial outer membrane integrity and for detection of mitochondria in subcellular fractions.
This kit is sufficient for 100 tests (96 well plate format).
5X Assay Buffer: 10 ml
2X Enzyme Dilution Buffer: 10 ml
Cytochrome c: 0.5ml (5 mg)
0.5 M Dithiothreitol (DTT): 0.1 ml
Cytochrome c Oxidase(positive control): 0.5 ml (1 vial)
10% Permeable reagent: 1 ml
Reagents and Equipment Required but Not Provided.
- 1 ml Cuvettes
- Analytical balance
- Ultrapure water
The kit ships on wet ice and storage at –20 °C is recommended. When stored unopened, the components in this kit are stable for 24 months. After initial thawing of the 0.5 M Dithiothreitol Solution, divide the solution into undiluted working aliquots (still at 0.5 M concentration) and store at –20 °C.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.