Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transduction molecule, FADD, and is activated early in apoptosis and is involved in the proteolysis and activation of pro-caspase-3. The upstream sequence of the site recognized by active caspase-8, IETD (Ile-Glu-Thr-Asp), is utilized as a basis for the highly specific caspase-8 substrate, Ac-IETD-AFC, and the caspase-8 inhibitor Z-IETD-FMK. Z-IETD-FMK(654 Daltons) is an irreversible and cell permeable inhibitor of caspase-8. This inhibitor can be used to inhibit caspase-8 activity and to study events downstream of caspase-8 activation.
1mg, 5 mg
Store undiluted at -20°C. Reconstitute the substrate before use. Reconstitute in 1 ml DMSO to yield 1 mg/ml peptide in DMSO. Store the reconstituted substrate at –20°C for up to 1-2 months and avoid repeated freeze-thaw cycles.
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