Protocols
Protocols
For the best possible results, Celltechgen strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at Celltechgen and ensure accurate and reproducible results.
Western Blotting & Immunoprecipitation
Western Immunoblotting Protocol (Western)
Western Immunoblotting (Fluorescent) Protocol (Fluorescent Western)
Western Blot Reprobing Protocol (Western / Reprobe)
Immunoprecipitation Protocol / (For Denatured Proteins) (IP / Denatured Protein)
Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein)
Immunoprecipitation Protocol Utilizing Magnetic Separation / (For Analysis By Western Immunoblotting) (IP Magnetic)
Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Immobilized IP)
Western Blotting Troubleshooting
Western Immunoblotting Troubleshooting Guide
Changes to Recommended Western Blotting Protocols
Immunohistochemistry
Fluorescent Multiplex Immunohistochemistry Protocol (mIHC/Paraffin)
Immunohistochemistry Troubleshooting
Immunohistochemistry Troubleshooting Guide
Immunofluorescence
Immunofluorescence Protocol (IF Standard)
Immunofluorescence Protocol with Methanol Fixation
Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm)
Flow Cytometry
Flow Cytometry Protocol (Flow)
Flow Cytometry Troubleshooting
Flow Cytometry Troubleshooting Guide
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation Troubleshooting
Chromatin Immunoprecipitation Troubleshooting Guide
Sandwich Immunoassays (ELISA)
ELISA-Peptide Assay Protocol (ELISA-P)
HRP Chemiluminescence ELISA-P (HRP Chemiluminescent ELISA-P)