Product Description
Normal mammalian cells divide for a limited number of population doublings and eventually enter an arrested state in which the cells remain alive, but do not proliferate in response to mitogens, and assume a characteristic enlarged, flattened morphology. This process is senescence and thought to be a tumor suppressive mechanism and underlying cause of aging. Senescence-associated β-galactosidase (SA- β-gal) is a widely used biochemical maker for assessing senescence in cultured cells. The Cell Senescence Assay provide an easy-to-use method to detect SA-β-gal by staining cells with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) at pH 6.0, a pH condition that suppress lysosomal β-galactosidase activity sufficiently to ensure that nonsenescent cells remain unstained.
Kit Size
50 Tests in 35 mm plate
Application
Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.
Kit Components
- 10X Fixation Buffer: 10 ml
- Reagent A: 1 ml Potassium Ferricyanide
- Reagent B: 1 ml Potassium Ferrocyanide
- X-gal Solution: 80 mg
- 10X Staining Solution: 10 ml
- 10X Phosphate Buffered Saline (PBS): 30 ml
Additional Reagents (Not Supplied)
- DMF (Dimethylformamide)
- Polypropylene tubes
- 37°C dry incubator (No CO2 )
- Phase contrast or light microscope
- 70% glycerol (optional)
Kit storage/stability
Store X-gal solution protected from light at -20ºC. Store all other components at 4ºC.
Usage
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
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