Normal mammalian cells divide for a limited number of population doublings and eventually enter an arrested state in which the cells remain alive, but do not proliferate in response to mitogens, and assume a characteristic enlarged, flattened morphology. This process is senescence and thought to be a tumor suppressive mechanism and underlying cause of aging. Senescence-associated β-galactosidase (SA- β-gal) is a widely used biochemical maker for assessing senescence in cultured cells. The Cell Senescence Assay provide an easy-to-use method to detect SA-β-gal by staining cells with 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) at pH 6.0, a pH condition that suppress lysosomal β-galactosidase activity sufficiently to ensure that nonsenescent cells remain unstained.
50 Tests in 35 mm plate
Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.
- 10X Fixation Buffer: 10 ml
- Reagent A: 1 ml Potassium Ferricyanide
- Reagent B: 1 ml Potassium Ferrocyanide
- X-gal Solution: 80 mg
- 10X Staining Solution: 10 ml
- 10X Phosphate Buffered Saline (PBS): 30 ml
Additional Reagents (Not Supplied)
- DMF (Dimethylformamide)
- Polypropylene tubes
- 37°C dry incubator (No CO2 )
- Phase contrast or light microscope
- 70% glycerol (optional)
Store X-gal solution protected from light at -20ºC. Store all other components at 4ºC.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.