Cross-linked Allophycocyanin (cl-APC)
Catalog Number: AX-AP03
A651/A280 >= 4.7
A651/A620 > 1.5
Cross linker rate: 1.2
Molecular Weight: 105 kDa
Absorption Maximum: 650 nm
Emission Maximum: 662 nm
Extinction Coefficient: 7.0 × 105 cm-1 M-1
Quantum Yield: 0.68
cl-APC is supplied in 100 mM Potassium Phosphate buffer, pH7.0 with 60 % Saturated (NH4)2SO4, 1 mM EDTA, and 1 mM Sodium Azide.
Allophycocyanin (APC), purified from Spirulina, is made up of alpha and beta subunits and is present as a trimer (αβ)3, which is unstable and susceptible to dissociation at low concentrations. The monomer, αβ, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is also shifted to 620 nm. The chemically cross-linked APC trimer is much more stable than the native APC trimer, but still retains the same spectroscopic properties as the native APC trimer.
Mix thoroughly cl-APC until no precipitate is observed to get homogeneous solution. Further removal all the remaining ammonium sulfate and buffer exchange to the conjugation buffer.
No preservative is added in the product. Once reconstitute it, please use it as soon as possible.
Upon receipt store at 4°C protected from light. Do not freeze. Product shipped with an ice pack. For research or further manufacturing use only.