Myeloperoxidase (MPO) Fluorescenic Activity Assay Kit is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay. In the MPO assay protocol, myeloperoxidase produces HClO from H2O2 and Cl–. Myeloperoxidase (MPO) is a peroxidase enzyme (EC 18.104.22.168) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In Celltechgen’s MPO fluorescenic Assay Kit, MPO catalyzes the production of NaClO from H₂O₂ and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 490/515 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 μU per well. Myeloperoxidase peroxidation activity, please check Celltechgen’s Myeloperoxidase Peroxidation Activity Assay Kit (Cat.No:CTG-AK0037)
MPO Fluorescenic Activity Assay Kit has been used to determine the activity of myeloperoxidase in samples.
MPO Assay Buffer 25 ml
MPO Substrate Stock 50 µl
MPO Probe 200 µl
Stop Mix 100 µl
MPO Positive Control 1 vial (MPO)
Fluorescein Standard (1 mM) 50 µL
Store kit at -20°C protected from light. Allow Assay Buffer to warm to room temperature
before use. Briefly centrifuge small vials before opening.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.